The Barcelona-based Pluripotency Laboratory is advancing
induced pluripotent stem cell (iPSC) technology for iPSC closer to ground state
pluripotency for study into neural, eye and cardiac disease as well as clinical applications. We are part of the University of Barcelona’s Faculty of Medicine.
Dr. Michael Edel
Aims of the Pluripotency Laboratory
- We aim to understand the role of cell cycle and epigenetic genes in attaining ground state pluripotency.
- Modeling neural diseases and injury: Spinal Muscular Atrophy (SMA) and Spinal Crod Injury (SCI).
- Furthermore we aim to define efficient differentiation protocols of iPS towards progenitor stem cells of various tissues.
- Finally, we aim to address a number of bottlenecks in the field such as the threat of genetic instability and to define clinical cell culture conditions for eventual cell replacement therapy for different types of human disease.
The research involves making clinical grade induced pluripotent stem cells i(PS cells) using modified RNA methods, as a model to study human disease (cellular level, genetical level and drug screening) for:
Spinal cord injury (Oligodendrocyte precursor cells)
Spinal Muscular Atrophy (Motor Neurons)
Cardiac diseases (beating Cardiomyocte cells)
Eye Disease (Limbal stem cells for occular surface damage).
Laboratory and core facilities available
We have a 100m2 fully equipped laboratory for molecular cell biology with two dedicated cell culture hoods for pluripotent stem cell and adult stem cell culture. In addition, through IDIBAPS we have access to core facility technical platforms for:
Genome array analysis
Universitat de Barcelona
Facultat de Medicina
Departament de Ciències Fisiològiques I
Tel & Fax: 93 403 52 78